With the year’s support of the Lower Mekong Initiative (LMI), Southeast Asia One Health University Network (SEAOHUN) in collaboration with Thailand One Health University Network (THOHUN) successfully fostered the establishment of Lao One Health University Network (LAOHUN), the newest country network of SEAOHUN. The LAOHUN comprises five universities and 18 faculties. The LAOHUN aims to become a collaborative network of universities fostering multi-sectoral effort on One Health (OH) workforce and policy development. Its mission is to develop a university network with the capacity to promote One Health awareness, knowledge, and practice through the expansion of universities, strengthening the network, and the advancement of the One Health workforce.